Product Name :
USP28 Mouse Monoclonal Antibody [A1F9]
Predicted band size :
122 kDa
Observed band size :
140 kDa
Synonyms:
Deubiquitinating enzyme 28 antibody KIAA1515 antibody Ubiquitin carboxyl terminal hydrolase 28 antibody Ubiquitin carboxyl-terminal hydrolase 28 antibody ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody Ubiquitin specific peptidase 28 antibody Ubiquitin specific processing protease 28 antibody Ubiquitin specific protease 28 antibody Ubiquitin thioesterase 28 antibody Ubiquitin thiolesterase 28 antibody 展开
Function :
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.(S)-H8-BINAP Purity Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.2-(Aminooxy)ethanamine dihydrochloride site
Antibody Type:
Mouse Monoclonal Antibody
Immunogen :
Recombinant protein within Human USP28 aa 509-860 / 1,077.
Species reactivity:
Human, Rat
Verify application:
WB
Coupling :
unconjugated
Clone number:
A1F9
RRID:
AB_3068891
Form :
Liquid
Concentration :
2ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:33602077 Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG1
Purification method :
Protein G affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 122 kDa
Positive control :
HeLa cell lysate, A431 cell lysate, 293T cell lysate, SW480 cell lysate, LNCaP cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, rat testis tissue lysate.